<?xml version='1.0' encoding='UTF-8'?>
<ProteomeXchangeDataset id="PXD038465" formatVersion="1.4.0" xsi:noNamespaceSchemaLocation="proteomeXchange-1.4.0.xsd" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <CvList>
    <Cv id="MS" fullName="PSI-MS" uri="https://raw.githubusercontent.com/HUPO-PSI/psi-ms-Cv/master/psi-ms.obo"/>
    <Cv id="MOD" fullName="PSI-MOD" uri="https://raw.githubusercontent.com/HUPO-PSI/psi-mod-CV/master/PSI-MOD.obo"/>
    <Cv id="UNIMOD" fullName="UNIMOD" uri="http://www.unimod.org/obo/unimod.obo"/>
  </CvList>
  <DatasetSummary announceDate="2023-06-27" hostingRepository="PanoramaPublic" title="Monitoring extended stable isotope-labeled synthetic peptides as a quality control for protease digestion in targeted, multiplexed mass spectrometry-based assays">
    <Description>Targeted mass spectrometry-based assays are proven to offer sensitive, selective quantification of proteins in a variety of translational applications, including cell lines, plasma/serum, dried blood spots and tissue biopsies. Recent efforts have successfully transferred targeted mass spectrometry methodologies, specifically multiplexed multiple reaction monitoring (MRM)-based assays, into the clinical environment. Yet, if MRM-based assays are to be utilized in the clinical space, quality control (QC) metrics are needed to control specifically for protease digestion, the sample processing step with the highest variability, thus ensuring optimal, reliable, and reproducible protein quantification. In this study, we evaluate the utility of monitoring extended stable isotope labeled internal standards (SIS) that are digested in situ within native protein lysates to survey the extent of sample protease digestion. Over 500 extended SIS peptides corresponding to targets of two separate multiplexed MRM assays, a direct-MRM and an immuno-MRM assay, were spiked into cell lysates which then underwent a series of systematically designed robustness experiments that stressed efficient protease digestion, including a trypsin digestion time-course as well as different protease digestion stressors. By simultaneously measuring levels of the extended and tryptic forms of the SIS peptide, the peak area ratio of extended SIS (hE) to tryptic SIS (hT), or hE/hT, was shown to effectively monitor perturbations in digestion performance. From time-course experiments, observed rates of extended SIS peptide digestion were calculated and hE/hT ratios uncovered an optimum time needed for protease digestion. When the endogenous peptides were quantified in these stressor and time-course experiments, this hE/hT QC metric reflected the sensitivity and fidelity of the MRM assays. This study demonstrates the effectiveness of monitoring extended SIS peptides within targeted, multiplexed MRM assays as a QC metric for protease digestion to ensure reliable, robust quantification of the endogenous protein targets.</Description>
    <ReviewLevel>
      <cvParam cvRef="MS" accession="MS:1002855" name="Non peer-reviewed dataset"/>
    </ReviewLevel>
    <RepositorySupport>
      <cvParam cvRef="MS" accession="MS:1002856" name="Supported dataset by repository"/>
    </RepositorySupport>
  </DatasetSummary>
  <DatasetIdentifierList>
    <DatasetIdentifier>
      <cvParam cvRef="MS" accession="MS:1001919" name="ProteomeXchange accession number" value="PXD038465"/>
      <cvParam cvRef="MS" accession="MS:1001921" name="ProteomeXchange accession number version number" value="1"/>
    </DatasetIdentifier>
    <DatasetIdentifier>
      <cvParam cvRef="MS" accession="MS:1002872" name="Panorama Public dataset identifier" value="https://panoramaweb.org/Extended_peptide_MRM_digestion_QC.url"/>
    </DatasetIdentifier>
  </DatasetIdentifierList>
  <DatasetOriginList>
    <DatasetOrigin>
      <cvParam cvRef="MS" accession="MS:1002868" name="Original data"/>
    </DatasetOrigin>
  </DatasetOriginList>
  <SpeciesList>
    <Species>
      <cvParam cvRef="MS" accession="MS:1001469" name="taxonomy: scientific name" value="Homo sapiens"/>
      <cvParam cvRef="MS" accession="MS:1001467" name="taxonomy: NCBI TaxID" value="9606"/>
    </Species>
  </SpeciesList>
  <InstrumentList>
    <Instrument id="instrument_1">
      <cvParam cvRef="MS" accession="MS:1002581" name="QTRAP 6500"/>
    </Instrument>
    <Instrument id="instrument_2">
      <cvParam cvRef="MS" accession="MS:1000931" name="QTRAP 5500"/>
    </Instrument>
  </InstrumentList>
  <ModificationList>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:121" name="GG"/>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:21" name="Phospho"/>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:35" name="Oxidation"/>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:4" name="Carbamidomethyl"/>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:1297" name="Label:13C(3)15N(1)"/>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:695" name="Label:13C(6)15N(1)"/>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:259" name="Label:13C(6)15N(2)"/>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:269" name="Label:13C(9)15N(1)"/>
    <cvParam cvRef="UNIMOD" accession="UNIMOD:267" name="Label:13C(6)15N(4)"/>
  </ModificationList>
  <ContactList>
    <Contact id="lab_head">
      <cvParam cvRef="MS" accession="MS:1002332" name="lab head"/>
      <cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Jeff Whiteaker"/>
      <cvParam cvRef="MS" accession="MS:1000589" name="contact email" value="jwhiteak@fhcrc.org"/>
      <cvParam cvRef="MS" accession="MS:1000590" name="contact affiliation" value="Fred Hutchinson Cancer Center"/>
    </Contact>
    <Contact id="dataset_submitter">
      <cvParam cvRef="MS" accession="MS:1002037" name="dataset submitter"/>
      <cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Jacob Kennedy"/>
      <cvParam cvRef="MS" accession="MS:1000589" name="contact email" value="jkennedy@fhcrc.org"/>
      <cvParam cvRef="MS" accession="MS:1000590" name="contact affiliation" value="Fred Hutchinson Cancer Center"/>
    </Contact>
  </ContactList>
  <PublicationList>
    <Publication id="unpublished">
      <cvParam cvRef="MS" accession="MS:1002853" name="Dataset with no associated published manuscript"/>
    </Publication>
  </PublicationList>
  <KeywordList>
    <cvParam cvRef="MS" accession="MS:1001925" name="submitter keyword" value="MRM"/>
    <cvParam cvRef="MS" accession="MS:1001925" name="submitter keyword" value="immuno-affinity enrichment"/>
    <cvParam cvRef="MS" accession="MS:1001925" name="submitter keyword" value="quantification"/>
    <cvParam cvRef="MS" accession="MS:1001925" name="submitter keyword" value="human cell line"/>
    <cvParam cvRef="MS" accession="MS:1001925" name="submitter keyword" value="sample processing"/>
    <cvParam cvRef="MS" accession="MS:1001925" name="submitter keyword" value="QC"/>
  </KeywordList>
  <FullDatasetLinkList>
    <FullDatasetLink>
      <cvParam cvRef="MS" accession="MS:1002873" name="Panorama Public dataset URI" value="https://panoramaweb.org/Extended_peptide_MRM_digestion_QC.url"/>
    </FullDatasetLink>
  </FullDatasetLinkList>
</ProteomeXchangeDataset>