Update information. Aberrant activation of sonic hedgehog (SHH) signaling is critical for oncogenesis of SHH-dependent medulloblastoma (MBSHH) and basal cell carcinoma (BCC). Here, we quanti-tatively characterized phosphoproteomic changes in an GFAP-Cre;SMO-M2 and Smo-M2 control transgenic mouse using high-resolution mass spectrometry, profiled 6692 phospphorylation sites on 2553 proteins. Our results, revealed that 142 differentially expressed phospho-proteins were significantly upregulated, such as heterogeneous nuclear ribonucleoprotein C (Hnrnpc), protein kinase C  (PKC), PKC, and autophagy-related protein 9A (Atg9a), and 86 differentially expressed phospho-proteins were significantly down-regulated, such as DNA polymerase 2 (Pola2), nucleoporin 210 (Nup210), and p38 and MAPKs. Our phosphoproteome data facilitated the construction of detailed molecular landscape in MB and should serve as a resource for the brain tumors community.