PXD038148

PXD038148 is an original dataset announced via ProteomeXchange.

Title	IP-MS of human YKT6 upon mutation of possible ULK1-phosphorylation sites
Description	Autophagy is a catabolic process during which cytosolic material is enwrapped in a newly formed double membrane structure called the autophagosome, and subsequently targeted for degradation in the lytic compartment of the cell. The fusion of autophagosomes with the lytic compartment is a tightly regulated step and involves membrane-bound SNARE proteins. These play a crucial role as they promote lipid mixing and fusion of the opposing membranes. Among the SNARE proteins implicated in autophagy, the essential SNARE protein YKT6 is the only evolutionary conserved SNARE protein from yeast to human. Alterations in YKT6 function, in both mammalian cells and nematodes, produces early and late autophagy defects that result in reduced survival. Moreover, mammalian autophagosomal YKT6 is phospho-regulated by the ULK1 kinase, preventing premature bundling with the lysosomal SNARE proteins and autophagosome-lysosome fusion. Together, our findings reveal that timely regulation of theYKT6 phosphostatus is crucial throughout autophagy progression and cell survival.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:12:33.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georg Tascher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-15 07:10:55	ID requested
1	2023-03-02 02:00:03	announced
⏵ 2	2023-11-14 08:12:34	announced	2023-11-14: Updated project metadata.

S, á, nchez-Mart, í, n P, Kriegenburg F, Alves L, Adam J, Elsaesser J, Babic R, Mancilla H, Licheva M, Tascher G, M, ü, nch C, Eimer S, Kraft C, ULK1-mediated phosphorylation regulates the conserved role of YKT6 in autophagy. J Cell Sci, 136(3):(2023) [pubmed]

submitter keyword: Human, Q Exactive HF, HeLa, IP-MS, YKT6, LFQ

Claudine Kraft
contact affiliation	Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany
contact email	kraft@biochemie.uni-freiburg.de
lab head
Georg Tascher
contact affiliation	Institute of Biochemistry II, Goethe University Hospital Frankfurt/Main, Germany
contact email	tascher@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038148

PRIDE project URI

• PRIDE
  1. PXD038148
     1. Label: PRIDE project
     2. Name: IP-MS of human YKT6 upon mutation of possible ULK1-phosphorylation sites