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PXD038148 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIP-MS of human YKT6 upon mutation of possible ULK1-phosphorylation sites
DescriptionAutophagy is a catabolic process during which cytosolic material is enwrapped in a newly formed double membrane structure called the autophagosome, and subsequently targeted for degradation in the lytic compartment of the cell. The fusion of autophagosomes with the lytic compartment is a tightly regulated step and involves membrane-bound SNARE proteins. These play a crucial role as they promote lipid mixing and fusion of the opposing membranes. Among the SNARE proteins implicated in autophagy, the essential SNARE protein YKT6 is the only evolutionary conserved SNARE protein from yeast to human. Alterations in YKT6 function, in both mammalian cells and nematodes, produces early and late autophagy defects that result in reduced survival. Moreover, mammalian autophagosomal YKT6 is phospho-regulated by the ULK1 kinase, preventing premature bundling with the lysosomal SNARE proteins and autophagosome-lysosome fusion. Together, our findings reveal that timely regulation of theYKT6 phosphostatus is crucial throughout autophagy progression and cell survival.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGeorg Tascher
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-15 07:10:55ID requested
12023-03-02 02:00:03announced
22023-11-14 08:12:34announced2023-11-14: Updated project metadata.
Publication List
S, á, nchez-Mart, í, n P, Kriegenburg F, Alves L, Adam J, Elsaesser J, Babic R, Mancilla H, Licheva M, Tascher G, M, ü, nch C, Eimer S, Kraft C, ULK1-mediated phosphorylation regulates the conserved role of YKT6 in autophagy. J Cell Sci, 136(3):(2023) [pubmed]
Keyword List
submitter keyword: Human, Q Exactive HF, HeLa, IP-MS, YKT6, LFQ
Contact List
Claudine Kraft
contact affiliationInstitute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany
contact emailkraft@biochemie.uni-freiburg.de
lab head
Georg Tascher
contact affiliationInstitute of Biochemistry II, Goethe University Hospital Frankfurt/Main, Germany
contact emailtascher@med.uni-frankfurt.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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