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PXD038132 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemoproteomic discovery of a human RNA ligase
DescriptionRNA ligases are present across all forms of life. While enzymatic RNA ligation between 5'-PO4 and 3'-OH termini is prevalent in viruses, fungi, and plants, such RNA ligases are yet to be identified in vertebrates. Here, using a nucleotide-based chemical probe targeting human AMPylated proteome, we have enriched and identified the hitherto uncharacterised human protein chromosome 12 open reading frame 29 (C12orf29) as the first human enzyme promoting RNA ligation between 5'-PO4 and 3'-OH termini. C12orf29 catalyses ATP-dependent RNA ligation via a three-step mechanism, involving tandem auto- and RNA AMPylation. Knock-out of C12ORF29 gene impedes the cellular resilience to oxidative stress featuring concurrent RNA degradation, which suggests a role of C12orf29 in maintaining RNA integrity. This data provides the groundwork for establishing a human RNA repair pathway.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; adenylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-14 02:02:50ID requested
12023-01-26 23:56:57announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: RNA ligation, AMPylation,Proteomics, Post-translational modification
Contact List
contact affiliationOrganic Chemistry/Cellular Chemistry, Department of Chemistry, University of Konstanz
contact emailandreas.marx@uni-konstanz.de
lab head
contact affiliationUniversity of Konstanz
contact emailphilip.saumer@uni-konstanz.de
dataset submitter
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Dataset FTP location
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