PXD038067-2

PXD038067 is an original dataset announced via ProteomeXchange.

Title	Proximity labelling and LCMSMS analysis of spliceosome complexes using SUGP1-miniTurbo
Description	The purpose of the project is to identify proteins binding differentially to the human specific spliceosomal protein SUGP1 in the context of point mutations to its G-patch domain. SUGP1 wt and mutant (G603N or L570E) C-terminally tagged with mini-Turbo-NLS was overexpressed for 48 h in HEK293T cells prior to a 2 min labelling pulse with 200 µM biotin. A non-transfection control was included and all samples were prepared in four independent biological replicates. Biotinylated proteins were captured by incubating total cell lysates for 30 min at 4 °C with streptavidin beads. The enrichment was then performed exactly as described in Cho et al. Nat Protoc 15, 3971–3999 (2020) pmid: 33139955
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:41.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Oses-Prieto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-09 10:32:57	ID requested
1	2023-10-05 10:00:29	announced
⏵ 2	2023-11-14 09:05:41	announced	2023-11-14: Updated project metadata.

Beusch I, Rao B, Studer MK, Luhovska T, Š, ukyt, ė V, Lei S, Oses-Prieto J, SeGraves E, Burlingame A, Jonas S, Madhani HD, vivo operating principles. Mol Cell, 83(14):2578-2594.e9(2023) [pubmed]

submitter keyword: Spliceosome, SUGP1, Proximity labeling, miniTurbo, BioID

Hiten D.
contact affiliation	Professor & Vice-Chair Dept. of Biochemistry and Biophysics UCSF
contact email	hitenmadhani@gmail.com
lab head
Juan Oses-Prieto
contact affiliation	UCSF
contact email	joses@cgl.ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038067

PRIDE project URI

• PRIDE
  1. PXD038067
     1. Label: PRIDE project
     2. Name: Proximity labelling and LCMSMS analysis of spliceosome complexes using SUGP1-miniTurbo