PXD038067 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximity labelling and LCMSMS analysis of spliceosome complexes using SUGP1-miniTurbo |
Description | The purpose of the project is to identify proteins binding differentially to the human specific spliceosomal protein SUGP1 in the context of point mutations to its G-patch domain. SUGP1 wt and mutant (G603N or L570E) C-terminally tagged with mini-Turbo-NLS was overexpressed for 48 h in HEK293T cells prior to a 2 min labelling pulse with 200 µM biotin. A non-transfection control was included and all samples were prepared in four independent biological replicates. Biotinylated proteins were captured by incubating total cell lysates for 30 min at 4 °C with streptavidin beads. The enrichment was then performed exactly as described in Cho et al. Nat Protoc 15, 3971–3999 (2020) pmid: 33139955 |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:05:41.097.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Juan Oses-Prieto |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-09 10:32:57 | ID requested | |
1 | 2023-10-05 10:00:29 | announced | |
⏵ 2 | 2023-11-14 09:05:41 | announced | 2023-11-14: Updated project metadata. |
Publication List
Beusch I, Rao B, Studer MK, Luhovska T, Š, ukyt, ė V, Lei S, Oses-Prieto J, SeGraves E, Burlingame A, Jonas S, Madhani HD, vivo operating principles. Mol Cell, 83(14):2578-2594.e9(2023) [pubmed] |
Keyword List
submitter keyword: Spliceosome, SUGP1, Proximity labeling, miniTurbo, BioID |
Contact List
Hiten D. |
contact affiliation | Professor & Vice-Chair Dept. of Biochemistry and Biophysics UCSF |
contact email | hitenmadhani@gmail.com |
lab head | |
Juan Oses-Prieto |
contact affiliation | UCSF |
contact email | joses@cgl.ucsf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038067 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038067
- Label: PRIDE project
- Name: Proximity labelling and LCMSMS analysis of spliceosome complexes using SUGP1-miniTurbo