PXD038018

PXD038018 is an original dataset announced via ProteomeXchange.

Title	Deciphering intercellular signaling complexes by interaction-guided chemical proteomics
Description	Indirect cell-cell interactions mediated by secreted proteins and their plasma membrane receptors play essential roles for regulating intercellular signaling. However, systematic profiling of the interactions between living cell surface receptors and secretome from neighboring cells remains challenging. Here we developed a chemical proteomics approach, termed interaction-guided crosslinking (IGC), to identify ligand-receptor interactions in situ. By introducing glycan-based ligation and click chemistry, the IGC approach via glycan-to-glycan crosslinking successfully captured receptors from as few as 0.1 million living cells using only 10 ng of secreted ligand. The unparalleled sensitivity and selectivity allow systematic crosslinking and identification of ligand-receptor complexes formed between cell secretome and surfaceome in an unbiased and all-to-all manner, leading to the discovery of a novel ligand-receptor interaction between pancreatic cancer cell-secreted urokinase (PLAU) and neuropilin 1 (NRP1) on pancreatic cancer-associated fibroblasts. This approach is thus useful for systematic exploring new ligand-receptor pairs and discovering critical intercellular signaling events.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:04:26.894.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiangnan Zheng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; timsTOF Pro; Q Exactive; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-07 10:08:49	ID requested
1	2023-07-07 21:17:35	announced
⏵ 2	2023-11-14 07:04:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: LC-MS/MS

Ruijun Tian
contact affiliation	Southern University of Science and Technology
contact email	tianrj@sustech.edu.cn
lab head
Jiangnan Zheng
contact affiliation	Southern University of Science and Technology
contact email	zjnlive@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038018
     1. Label: PRIDE project
     2. Name: Deciphering intercellular signaling complexes by interaction-guided chemical proteomics