Cocoonase is a proteolytic enzyme secreted by tasar silk moth during emergence. Amino acid sequence of Antheraea mylitta cocoonase is unavailable in uniprot database. Therefore, an attempt was made to sequence cocoonase. The amino acid information will help in to know the properties of cocoonase, predict its structure and many more information can be generated. The amino acid sequence of Antheraea mylitta cocoonase was also compared with the cocoonase of other non-sericigenous organisms.