<<< Full experiment listing


PXD037910 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMouse osteoclast homogenates nLC-ESI-MS/MS - WT vs Slc37a2 KO
DescriptionTo survey the proteomic differences between WT and Slc37a2 knockout osteoclasts at the whole cell and secretory lysosome levels, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed’ with SPIONs to encourage uptake into endosomes and then ‘chased’ into secretory lysosomes upon the convergence of SPION-loaded endosomes with lysosomes and secretory pathways. Following the ‘pulse-chase’, osteoclasts were homogenized, SPION-loaded organelles captured-from post-nuclear supernatants using magnetic columns, and enriched organelles as well as homogenates eluted and processed for 1D in-gel digestion and mass spectrometry. The samples presented here correspond to the proteome of WT and Slc37a2 knockout homogenates with secretory lysosome proteomes have been shared in another submission.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-03 04:22:40ID requested
12023-01-24 19:45:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Lysosome, LRO, Mouse, SPION,Osteoclast, Secretory lysosome
Contact List
contact affiliationThe Bone Biology and Disease Laboratory, School of Biomedical Sciences, The University of Western Australia
contact emailnathan.pavlos@uwa.edu.au
lab head
contact affiliationThe University of Western Australia
contact email21703693@student.uwa.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD037910
PRIDE project URI
Repository Record List
[ + ]