Samples were injected into Thermo ScientificTM DionexTM UltimateTM 3000 RSLCnano systems equipped with NCS-3500RS (microflow) module and Q Exactive Plus – OrbitrapTM spectrometer (Thermo Fisher Scientific) operated in Data-Independent Acquisition (DIA) mode for peptide and protein quantification. Raw data was processed and analyzed by DIA-NN software using default parameters. Q value (FDR) cut-off on precursor and protein level was applied 1%. All selected precursors that passed the filters were used for quantification.