PXD037806

PXD037806 is an original dataset announced via ProteomeXchange.

Title	Integrating intracellular and extracellular proteomic profiling for in-depth investigations of cellular communication in a model of prostate cancer
Description	Cellular communication is essential for cell-cell interactions, maintaining homeostasis and progression of certain disease states. While many studies examine extracellular proteins, the holistic extracellular proteome is often left uncaptured, leaving gaps in our understanding of how all extracellular proteins may impact communication and interaction. We used a cellular-based proteomics approach to more holistically profile both the intracellular and extracellular proteome of prostate cancer. Our workflow was generated in such a manner that multiple experimental conditions can be observed with the opportunity for high throughput integration. Additionally, this workflow is not limited to a proteomic aspect, as metabolomic and lipidomic studies can be integrated for a multi-omics workflow. Our analysis showed coverage of over 8000 proteins while also garnering insights into cellular communication in the context of prostate cancer development and progression. Identified proteins covered a variety of cellular processes and pathways, allowing for investigation of multiple aspects into cellular biology. This workflow demonstrates advantages for integrating intra- and extracellular proteomic analyses as well as potential for multi-omics researchers. This approach possesses great value for future investigations into the systems biology aspects of disease development and progression.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:31.037.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannah Miles
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-28 07:35:31	ID requested
1	2023-05-25 05:38:41	announced
2	2023-06-02 07:38:50	announced	2023-06-02: Updated project metadata.
⏵ 3	2023-11-14 08:48:32	announced	2023-11-14: Updated project metadata.

Miles HN, Tomlin D, Ricke WA, Li L, Integrating intracellular and extracellular proteomic profiling for in-depth investigations of cellular communication in a model of prostate cancer. Proteomics, 23(21-22):e2200287(2023) [pubmed]

submitter keyword: Prostate cancer, extracellular proteomics, LC-MS/MS

Lingjun Li
contact affiliation	Department of Chemistry and School of Pharmacy, University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Hannah Miles
contact affiliation	UW-Madison
contact email	hnmiles@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037806

PRIDE project URI

• PRIDE
  1. PXD037806
     1. Label: PRIDE project
     2. Name: Integrating intracellular and extracellular proteomic profiling for in-depth investigations of cellular communication in a model of prostate cancer