PXD037460

PXD037460 is an original dataset announced via ProteomeXchange.

Title	NLRP6 controls PTEN stability by promoting autophagic degradation of p85α to drive tumorigenesis
Description	To identify NLRP6-associated proteins in living cells, we applied an APEX2-based labelling method combined with mass spectrometry. In brief, APEX2 was genetically fused to NLRP6. In the presence of hydrogen peroxide, APEX2 catalysed biotin-phenol to become a biotin-phenol radical that covalently bound to NLRP6 neighbouring proteins (<20 nm). The biotinylated proteins were enriched and collected by streptavidin beads. One-dimensional SDS–PAGE followed by liquid chromatography-tandem mass spectrometry (GeLC–MS/MS) was performed to identify the proteins. To detect ubiquitinated p85α, a Flag-tagged p85α plasmid was transfected into cells. The cell lysates were immunoprecipitated with anti-Flag and separated on a 6% SDS–PAGE gel. After in-gel digestion, the resulting peptides were extracted for GeLC–MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2023-07-02
AnnouncementXML	Submission_2023-07-01_22:39:29.943.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BowenLi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-14 14:53:36	ID requested
⏵ 1	2023-07-01 22:39:30	announced

Dataset with its publication pending

submitter keyword: Human,LC-MS/MS

JunCui
contact affiliation	Sun Yat-sen University
contact email	cuij5@mail.sysu.edu.cn
lab head
BowenLi
contact affiliation	Third Affiliated Hospital of Soochow University
contact email	libowen2012@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD037460

PRIDE project URI

• PRIDE
  1. PXD037460
     1. Label: PRIDE project
     2. Name: NLRP6 controls PTEN stability by promoting autophagic degradation of p85α to drive tumorigenesis