Updated project metadata. To assess the impact of the bactericidal compounds CymA and ecumicin on the mycobacterial proteome and to monitor proteome remodeling by the AAA+ ClpC1 protease machinery, we performed a LFQ proteomics analysis treating the cells at bactericidal compound concentration. We identified new players of the mycobacterial ClpC1 stress response (ClpC2 and ClpC3), as novel bacterial antibiotic resistance mechanism.