PXD037225

PXD037225 is an original dataset announced via ProteomeXchange.

Title	Surfaceome analysis of extracellular vesicles from senescent cells uncovers uptake repressor DPP4
Description	Senescent cells are beneficial for repairing acute tissue damage but are harmful when they accumulate in tissues, as occurs with advancing age. Senescence-associated extracellular vesicles (S-EVs) can mediate cell-to-cell communication and export intracellular content to the microenvironment of aging tissues. Here, we studied the uptake of EVs from senescent cells (S-EVs) relative to proliferating cells (P-EVs). Interestingly, P-EVs were readily taken up by other proliferating cells (fibroblasts and cervical cancer cells) while S-EVs were not; by contrast, macrophages selectively engulfed all EVs (P-EVs, S-EVs). We thus investigated the surface proteome (surfaceome) of P-EVs relative to S-EVs derived from cells that had reached senescence via replicative exhaustion, exposure to ionizing radiation, or treatment with etoposide. We discovered that relative to P-EVs, S-EVs from all senescence models were enriched in proteins DPP4, ANXA1, ANXA6, S10AB, AT1A1, and EPHB2. Among them, DPP4 was found to dictate selective uptake by proliferating cells, as ectopic overexpression of DPP4 in HeLa cells rendered DPP-expressing EVs that were no longer taken up by other proliferating cells. We propose that DPP4 on the surface of S-EVs makes these EVs refractory to internalization by proliferating cells, advancing our knowledge of the impact of senescent cells in aging-associated processes.
HostingRepository	MassIVE
AnnounceDate	2023-01-03
AnnouncementXML	Submission_2023-01-03_18:48:18.632.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Supriyo De
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-09 22:02:49	ID requested
⏵ 1	2023-01-03 18:48:19	announced

no publication

submitter keyword: Extracellular Vesicles, Surface Protein

Supriyo De
contact affiliation	National Institute on Aging Intramural Research Program, National Institutes of Health
contact email	desu@grc.nia.nih.gov
lab head
Myriam Gorospe
contact affiliation	National Institute on Aging Intramural Research Program, National Institutes of Health
contact email	gorospem@grc.nia.nih.gov
lab head
Supriyo De
contact affiliation	NIA IRP, NIH
contact email	niairpuser@mail.nih.gov
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090503/