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PXD037117

PXD037117 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTORC1 phosphorylates and inhibits the ribosome preservation factor Stm1 to activate dormant ribosomes
DescriptionTarget of rapamycin complex 1 (TORC1) promotes biogenesis and inhibits degradation of ribosomes in response to nutrient availability. To ensure a basal supply of ribosomes, cells preserve a small pool of dormant ribosomes under nutrient-limited conditions. The regulation of dormant ribosomes is poorly characterized. Here, we show that upon inhibition of TORC1 by rapamycin or nitrogen starvation, Stm1 (suppressor of target of Myb protein 1) forms non-translating, dormant 80S ribosomes. Furthermore, Stm1-bound 80S ribosomes are protected from proteasomal degradation. Upon re-feeding, TORC1 directly phosphorylates and inhibits Stm1, thereby reactivating translation. Finally, SERBP1 (SERPINE1 mRNA binding protein), a mammalian ortholog of Stm1, forms dormant 80S ribosomes upon mTORC1 inhibition in mammalian cells. Thus, TORC1 regulates ribosomal dormancy in an evolutionarily conserved manner via a ribosome preservation factor.
HostingRepositoryPRIDE
AnnounceDate2023-01-19
AnnouncementXMLSubmission_2023-01-19_03:04:50.821.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD037117
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDaniloRitz
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-30 04:10:27ID requested
12023-01-19 03:04:51announced
Publication List
10.6019/PXD037117;
Keyword List
submitter keyword: Protein Synthesis, Dormant Ribosome, Proteasome, TORC1, Ribosome preservation Factor, Ribophagy
Contact List
AlexanderSchmidt
contact affiliationProteomics Core Facility Biozentrum, Universit├Ąt Basel Spitalstrasse 41 CH - 4056 Basel Schweiz
contact emailalex.schmidt@unibas.ch
lab head
DaniloRitz
contact affiliationProteomics Core Facility Biozentrum, University of Basel Klingelbergstrasse 50/70 CH-4056 Basel / Switzerland
contact emaildanilo.ritz@unibas.ch
dataset submitter
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