PXD036850
PXD036850 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Unique position of ATG5 in the atg8ylation cascade provides a switch between autophagy and secretion |
| Description | ATG5 is a part of the E3 ligase directing lipidation of ATG8 proteins (mATG8s), a process central to membrane atg8ylation and its downstream homeostatic manifestations, including canonical autophagy, with impact on health. Genetic ablation of ATG5 in myeloid cells causes mortality in murine models of tuberculosis. Paradoxically, this in vivo phenotype is specific to ATG5 and does not apply to other ATGs. Here we show that absence of ATG5 but not of other components of canonical autophagy, promotes lysosomal exocytosis, secretion of extracellular vesicles, and in neutrophils their excessive degranulation. This is due to lysosomal disrepair in ATG5 knockout cells and sequestration of ESCRT proteins by an alternative ATG conjugation complex. These findings explain the unique nature of ATG5 in host-protective role in murine experimental models of tuberculosis, and emphasize the significance of the branching aspects of the atg8ylation conjugation cascade beyond the canonical autophagy. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-11-01 |
| AnnouncementXML | Submission_2023-11-01_11:10:09.594.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brett Phinney |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-09-19 16:19:18 | ID requested | |
| ⏵ 1 | 2023-11-01 11:10:09 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: atg8ylation,ATG5,E3 ligase,ATG8 proteins |
Contact List
| Vojo Deretic | |
|---|---|
| contact affiliation | University of New Mexico School of Medicine |
| contact email | vderetic@salud.unm.edu |
| lab head | |
| Brett Phinney | |
| contact affiliation | UC Davis |
| contact email | brettsp@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090348/ |
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