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PXD036761 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type-III effector
DescriptionPlant immunity is tightly controlled by a complex and dynamic regulatory network, which ensures optimal activation upon detection of potential pathogens. Accordingly, each component of this network is a potential target for manipulation by pathogens. Here, we report that RipAC, a type III-secreted effector from the bacterial pathogen Ralstonia solanacearum, targets the plant E3 ubiquitin ligase PUB4 to inhibit pattern-triggered immunity (PTI). PUB4 plays a positive role in PTI by regulating the homeostasis of the central immune kinase BIK1. Before PAMP perception, PUB4 promotes the degradation of non-activated BIK1, while, after PAMP perception, PUB4 contributes to the accumulation of activated BIK1. RipAC leads to BIK1 degradation, which correlates with its PTI-inhibitory activity. RipAC causes a reduction in pathogen-associated molecular pattern (PAMP)-induced PUB4 accumulation and phosphorylation. Our results shed light on the role played by PUB4 in immune regulation, and illustrate an indirect targeting of the immune signalling hub BIK1 by a bacterial effector.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterFrank Menke
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationListCarbamidomethyl; Oxidation; Phospho
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-15 19:31:55ID requested
12022-10-12 08:20:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plant immunity, phosphorylation, effector
Contact List
Frank Menke
contact affiliationSainsbury Laboratory
contact emailfrank.menke@tsl.ac.uk
lab head
Frank Menke
contact affiliationSainsbury Laboratory
contact emailfrank.menke@tsl.ac.uk
dataset submitter
Full Dataset Link List
Panorama Public dataset URI