Update information. Lipopolysaccharide (LPS) is a component of the outer membrane of gram-negative bacteria that is involved in pathogenic processes leading to postpartum metritis and endometritis in dairy cow. It also causes inflammation of the endometrium. This study aimed to investigate the effects of LPS on cow endometrial epithelial cells (BEECs). In this study, the label-free LC–MS/MS approach was employed to investigate the proteome profile of cow endometrial epithelial cells. A total of 1121 proteins were identified in the control group and model group, of which 186 differential proteins were accurately quantified. The proteomic results were verified by WB and IF techniques, and the same conclusion was obtained