This study hypothesized that adenine methylation was involved in bacterial regulation of physiological adaptations when grown under ethanol stress. Thus, this study compared the growth of an adenine methyltransferase-inactivated mutant (ΔpglX) and the wild-type L. paracasei Zhang strains when cultured under ethanol stress. Proteomic analyses was implemented to identify differentially expressed proteins (DEPs) of the bacteria.