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PXD035373

PXD035373 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry to screen potential ORMs-interacting proteins
DescriptionTo screen potential ORMs-interacting proteins, HEK293T cells were transfected with plasmids containing HA-tagged ORM1, HA-tagged ORM2, HA-tagged ORM3 and HA-Empty vector, respectively. Then, protein lysates were harvested for immunoprecipitation. Mass spectrometry combined with high-performance liquid chromatography (HPLC-MS) was performed by Cloud‐Seq Biotech (Shanghai, China) using Q Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Proteins were digested with trypsin (trypsin: protein = 1:50 (w/w)) at 37°C for 16 hours. After digestion, the protein fragments were separated by high pH reverse-phase HPLC. The peptides were then analyzed by an EASY-nLC 1000 system connected to an Q Exactive mass spectrometer and Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). In raw file, MaxQuant 1.6.0.16 was used to analyze the data, and MaxQuant own algorithm was used to identify peptides and proteins.
HostingRepositoryiProX
AnnounceDate2022-07-15
AnnouncementXMLSubmission_2022-07-17_20:05:32.413.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYan Lu
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-17 20:05:26ID requested
12022-07-17 20:05:32announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mass spectrometry, ORM1, ORM2, ORM3, Interacting proteins
Contact List
Yan Lu
contact affiliationZhongshan Hospital, Fudan University
contact emailrjluyan@126.com
lab head
Yan Lu
contact affiliationZhongshan Hospital, Fudan University
contact emailrjluyan@126.com
dataset submitter
Full Dataset Link List
iProX dataset URI