The zoonotic liver fluke Fasciola hepatica is responsible for economic losses to the agriculture industry worldwide. Triclabendazole has been the drug of choice. However, anthelmintic resistance is becoming prevalent with cases of resistance detected globally. This emphasizes the need for alternative control methods. A significant focus is on the development of protein vaccines. In this process however, glycoproteins (GPs) have been mostly ignored. GPs are present throughout the organ systems of F. hepatica and such gut-associated GPs could serve as ‘hidden’ antigens. Hidden antigens are not initially exposed to the host’s immune system during the course of natural infections, but have proven to be effective vaccine candidates, notably for antigens that are present in the intestine of blood-feeding and tissue-dwelling parasites. This work describes the purification of gut-associated parasite GPs based on protective antibodies against adult flukes in vitro, which demonstrate a binding on fluke gut structures. Finally, we provide a list of promising novel F. hepatica gut-associated vaccine candidates.