PXD035029 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The regulatory landscape of the yeast phosphoproteome – ultra-deep reference phosphoproteomic DDA data |
Description | We systematically exposed the yeast Saccharomyces cerevisiae to a wide array of 101 perturbations and generated a quantitative mass spectrometry-based atlas of early phosphoproteomic responses. Perturbations covered broad classes of environmental, chemical, and drug perturbations and systematically targeted diverse aspects of yeast cell biology. Importantly, most perturbations focused on capturing early signaling responses (5 min treatments). An ultra-deep yeast phosphoproteome was generated from a pooled sample of all 5 min perturbations by multi protease digestion, deep offline fractionations using two different peptide separation methods, and data-dependent acquisition (DDA). This submission includes 120 DDA-MS raw files that were used to generate a deep yeast phosphoproteome. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:01:50.877.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD035029 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Mario Leutert |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse; Orbitrap Exploris 480; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-30 07:17:51 | ID requested | |
1 | 2023-08-27 09:50:30 | announced | |
⏵ 2 | 2024-10-22 06:01:51 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Signaling,Phosphoproteomics, Orbitrap Exploris 480, Orbitrap Eclipse, PTM, Saccharomyces cerevisiae, Stress Response, DDA, Phosphorylation, Phosphatase, Budding Yeast, Fractionation, R2-P2, Kinase |
Contact List
Judit Villén |
contact affiliation | Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA |
contact email | jvillen@uw.edu |
lab head | |
Mario Leutert |
contact affiliation | Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA |
contact email | marioleutert@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD035029 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035029
- Label: PRIDE project
- Name: The regulatory landscape of the yeast phosphoproteome – ultra-deep reference phosphoproteomic DDA data