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PXD034342

PXD034342 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman mitochondria require mtRF1 for translation termination at non-canonical stop codons
DescriptionMitochondrial translation system highly diverged from its bacterial counterpart. This includes deviation from the universal genetic code, with AGA and AGG having no cognate tRNAs in human mitochondria. Their locations at the end of COX1 and ND6 open reading frames, respectively, suggests they function as stop codons. However, while canonical stop codons, UAA and UAG, are recognized by mtRF1a in mitochondria, the release mechanism at AGA and AGG remains a debated issue. Here, we show that upon the loss of another member of the mitochondrial release factor family, mtRF1, mitoribosomes accumulate specifically at AGA and AGG codons. Stalling of mitoribosomes alters COX1 transcript and protein levels, but not ND6 production. Finally, we set up an in vitro reconstituted mitochondrial translation system, which confirms the specific release activity of mtRF1 on AGA and AGG codons. Together, our study uncovers the mechanism of translation termination in mitochondria and provides first insights into the consequences of its failure.
HostingRepositoryPRIDE
AnnounceDate2022-11-16
AnnouncementXMLSubmission_2022-11-16_01:44:45.800.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIlian Atanassov
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-06 02:02:15ID requested
12022-11-16 01:44:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mtRF1, TMT, mitochondria, translation
Contact List
JoannaRorbach
contact affiliationDepartment of Medical Biochemistry and Biophysics, Division of Molecular Metabolism, Karolinska Institutet, Biomedicum, 171 65 Solna, Sweden. Max Planck Institute Biology of Ageing - Karolinska Institutet Laboratory, Karolinska Institutet, Stockholm, Sweden. 
contact emailJoanna.rorbach@ki.se
lab head
Ilian Atanassov
contact affiliationMax Planck Institute for Biology of Aging
contact emailIlian.Atanassov@age.mpg.de
dataset submitter
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Dataset FTP location
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