PXD034241

PXD034241 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS of Arabidopsis thaliana flower bud samples (TurboID-based proximity labeling of meiotic chromosome axes)
Description	The meiotic chromosome axis is a proteinaceous structure along which sister chromatids are arranged in a loop-base array during meiotic prophase I. In diverse species including plants, chromosome axis components have been shown to be critical for synapsis and meiotic recombination and hence essential for assuring the fidelity of meiosis. In Arabidopsis thaliana, the limited number of meiotic cells embedded in floral organs constrains proteomic approaches for the identification of new axis components. Therefore, we employed TurboID (TbID) based proximity labeling (PL) in meiotic cells of A. thaliana. The two axis proteins ASY1 and ASY3 were selected and ASY1-TbID and ASY3-TbID transgenic plants were generated to enable specific targeted biotin labeling of meiotic chromosome axis. ASY1-eYFP and nucTbID (TbID driven by UBQ10 promoter) were generated and used as controls. These lines were either treated or not with exogenous biotin followed by streptavidin affinity-purification coupled with mass spectrometry to identify their proximate candidate proteins. By stringent filtering a final candidates list was established which covered most of the known meiotic chromosome axis-related proteins and new proteins without a reported meiotic function. Functional validation of selected candidates and comparison with ASY1 AP-MS data (in Brassica oleracea) suggest the TbID based PL is a robust tool for identification of proximate proteins in meiotic cells of A. thaliana.
HostingRepository	PRIDE
AnnounceDate	2023-03-08
AnnouncementXML	Submission_2023-03-08_00:02:57.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RichardImre
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	ubiquitinylated lysine; biotinylated residue; phosphorylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-01 03:55:44	ID requested
⏵ 1	2023-03-08 00:02:58	announced

submitter keyword: Arabidopsis thaliana, LC-MSMS, Chromosome axes, Biotin, Proximity labeling, Meiosis, TurboID, Biotinylation, ASY1, ASY3, BioID

StefanHeckmann
contact affiliation	Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, D-06466 Seeland, Germany
contact email	heckmann@ipk-gatersleben.de
lab head
RichardImre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034241
     1. Label: PRIDE project
     2. Name: LC-MSMS of Arabidopsis thaliana flower bud samples (TurboID-based proximity labeling of meiotic chromosome axes)