In order to explore the underlying pathogenic role of Calsyntenin-1(CLSTN1) in dilated cardiomyopathy (DCM), we constructed cardiomyocyte-specific CLSTN1 overexpression (CLSTN1-OE) rats and a cumulative dose of 18 mg/kg doxorubicin were injected to induce DCM. Whole heart tissues from the Con-WT(n=4), Con-CLSTN1 OE(n=3), DCM-WT(n=3), and DCM-CLSTN1 OE(n=4) were collected for proteomics analysis via a QExactive mass spectrometer.