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PXD033694 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of the SF3B1 interactome
DescriptionCDK9 is a critical kinase required for the productive transcription of protein-coding genes by RNA polymerase II (pol II). As part of P-TEFb, CDK9 phosphorylates the carboxyl-terminal domain (CTD) of pol II and elongation factors, including SPT5, which allows pol II to elongate past the early elongation checkpoint (EEC) encountered soon after initiation. We show that, in addition to halting pol II at the EEC, loss of CDK9 activity causes premature termination of transcription across the last exon, loss of polyadenylation factors from chromatin, and loss of polyadenylation of nascent transcripts. Inhibition of the phosphatase PP2A abrogates the premature termination and loss of polyadenylation caused by CDK9 inhibition, indicating that this kinase/phosphatase pair regulates transcription elongation and RNA processing at the end of protein-coding genes. Our phosphoproteomic analyses after CDK9 inhibition, using either DRB or an analog-sensitive CDK9 cell line, confirm the splicing factor SF3B1 as an additional key target of this kinase. These results emphasize the important roles that CDK9 plays in coupling transcription elongation and termination to RNA maturation downstream of the EEC. As part of this project, we characterized the interactome of SF3B1 in HeLa cells in duplicate.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarjorie Fournier
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-05 01:03:52ID requested
12022-08-22 06:32:37announced
Publication List
Tellier M, Zaborowska J, Neve J, Nojima T, Hester S, Fournier M, Furger A, Murphy S, CDK9 and PP2A regulate RNA polymerase II transcription termination and coupled RNA maturation. EMBO Rep, 23(10):e54520(2022) [pubmed]
Keyword List
submitter keyword: SF3B1, splicing, transcription, interactome
Contact List
Shona Murphy
contact affiliationSir William Dunn School of Pathology, University of Oxford
contact emailshona.murphy@path.ox.ac.uk
lab head
Marjorie Fournier
contact affiliationUniversity of Oxford
contact emailmarjorie.fournier@bioch.ox.ac.uk
dataset submitter
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Dataset FTP location
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