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PXD033559 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA proteomics investigation of cigarette smoke exposed Wistar rats revealed improved anti-inflammatory effects of the cysteamine nanoemulsions delivered via inhalation
DescriptionCigarette smoking is the major cause of chronic inflammatory diseases such as Chronic Obstructive Pulmonary Disease (COPD). It is paramount to develop pharmacological interventions and delivery strategies against the cigarette smoke (CS) associated oxidative stress in COPD. This study in Wistar rats examined cysteamine in nanoemulsions to counteract the cigarette smoke distressed microenvironment. In vivo, 28 days of cigarette smoke and 15 days of cysteamine nanoemulsions treatment starting on 29th day consisting of oral and inhalation routes were established in Wistar rats. Additionally, we conducted inflammatory and epithelial-to-mesenchymal transition (EMT) studies in vitro in human bronchial epithelial cell lines (BEAS2B) using 5% cigarette smoke extract. Inflammatory and anti-inflammatory markers such as TNF-α, IL-6, IL-1ß, IL-8, IL-10, IL-13, have been quantified in bronchoalveolar lavage fluid (BALF) to evaluate the effects of the cysteamine nanoemulsions in normalizing the diseased condition. Histopathological analysis of the alveoli and the trachea showed the distorted, lung parenchyma and ciliated epithelial barrier, respectively. To obtain mechanistic insights into the cigarette smoke COPD rat model, “shotgun” proteomics of the lung tissues have been carried out using high-resolution mass spectrometry wherein genes such as ABI1, PPP3CA, PSMA2, FBLN5, ACTG1, CSNK2A1, and ECM1 exhibited significant differences across all the groups. Pathway analysis showed autophagy, signaling by receptor tyrosine kinase, cytokine signaling in immune system, extracellular matrix organization, and hemostasis, as the major contributing pathways across all the studied groups. This work offers new preclinical findings on how cysteamine taken orally or inhaled can combat cigarette smoke-induced oxidative stress.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterGautam Sharma
SpeciesList scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationListNo PTMs are included in the dataset
InstrumentTSQ Altis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-28 16:21:55ID requested
12023-08-23 10:45:33announced
Publication List
Sharma G, Pund S, Govindan R, Nissa MU, Biswas D, Middha S, Ganguly K, Anand MP, Banerjee R, Srivastava S, Inhalation. OMICS, 27(8):338-360(2023) [pubmed]
Keyword List
submitter keyword: Rat, COPD, cysteamine, MRM, proteomics
Contact List
Sanjeeva Srivastava
contact affiliationBiosciences and Bioengineering Department
contact emailsanjeeva@iitb.ac.in
lab head
Gautam Sharma
contact affiliationBiosciences and Bioengineering Department
contact emailgraviksharma45@gmail.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI