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PXD033557 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIron-Regulated Assembly of the Cytosolic Iron-Sulfur Cluster Biogenesis Machinery
DescriptionThe cytosolic iron-sulfur (Fe-S) cluster assembly (CIA) pathway delivers Fe-S clusters to nuclear and cytosolic Fe-S proteins involved in essential cellular functions. Although the delivery process is regulated by the availability of iron and oxygen, it remains unclear how CIA components orchestrate the cluster transfer under varying cellular environments. Here, we utilized a targeted proteomics assay for monitoring CIA factors and substrates to characterize the CIA machinery. We find that NUBP1 (NBP35), CIAO3 (NARFL) and CIA substrates associate with NUBP2 (CFD1), a component of the CIA scaffold complex. We also show that NUBP2 weakly associates with the CIA targeting complex (MMS19, CIAO1, CIAO2B) indicating the possible existence of a higher order complex. Interactions between CIAO3 and the CIA scaffold complex are strengthened upon iron supplementation or low oxygen tension, while iron chelation and reactive oxygen species weaken CIAO3 interactions with CIA components. We further demonstrate that CIAO3 mutants defective in Fe-S cluster binding fail to integrate into the higher order complexes. However, these mutants exhibit stronger associations with CIA substrates under conditions in which the association with the CIA targeting complex is reduced suggesting that CIAO3 and CIA substrates may associate in complexes independently of the CIA targeting complex. Together, our data suggest that CIA components potentially form a metabolon whose assembly is regulated by environmental cues and requires Fe-S cluster incorporation in CIAO3. These findings provide additional evidence that the CIA pathway adapts to changes in cellular environment through complex reorganization.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterXiaorui Fan
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-28 16:03:28ID requested
12022-06-06 09:14:54announced
Publication List
Fan X, Barshop WD, Vashisht AA, Pandey V, Leal S, Rayatpisheh S, Jami-Alahmadi Y, Sha J, Wohlschlegel JA, Iron-regulated assembly of the cytosolic iron-sulfur cluster biogenesis machinery. J Biol Chem, 298(7):102094(2022) [pubmed]
Keyword List
submitter keyword: PRM, iron-sulfur cluster, protein interactions
Contact List
James Wohlschlegel
contact affiliationUCLA Department of Biological Chemistry
contact emailjwohl@ucla.edu
lab head
Xiaorui Fan
contact affiliationUCLA Department of Biological Chemistry
contact emailxfbubu@gmail.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI