SARS-CoV-2 proteins orchestrate complex mechanisms that underlie SARS-CoV-2 infection and COVID-19 causing pathogenic processes. Here we have exhaustively defined human proteins in spatial proximity (proximal interaction network) of 27/28 SARS-CoV-2 proteins in human cells by proximity-dependent biotinylation (BioID)-based approach. We report 10,185 high-confidence virus-host PPI involving 2,592 unique cellular targets.