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PXD033395 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDifferential gene expression of cells located in the top and bottom of yeast colonies
DescriptionSaccharomyces cerevisiae colonies were grown on synthetic minimal media containing 1% glucose. The top layer of cells was scraped off using a custom plastic guide and a cell scraper. The remaining bottom layer of cells was then washed off with water and proteomics samples were prepared from both layers separately. Label-free quantification by DIA-SWATH was used to assess differences in gene expression between top and bottom layers.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-23 15:33:41ID requested
12023-02-13 04:28:39announced
Publication List
Keyword List
submitter keyword: DIA, SWATH, colony,yeast
Contact List
contact affiliationCharité Universitätsmedizin Berlin, Institute of Biochemistry
contact emailmarkus.ralser@charite.de
lab head
contact affiliationThe Francis Crick Institute
contact emailstephan.kamrad@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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