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PXD033364 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted proteomics (parallel reaction monitoring) to quantify starch-biosynthetic proteins in yeast
DescriptionHeterologous protein expression in Saccharomyces cerevisiae is a powerful testbed to dissect the functions of plant starch biosynthetic enzymes and create diverse starch-like polymers. Here we modulated the expression each starch synthase together with a branching enzyme (derived from Arabidopsis thaliana) in a suite of yeast strains and quantified protein abundances using targeted proteomics. Specifically, we developed parallel reaction monitoring assays to quantify the Arabidopsis proteins SS1, SS2, SS3, SS4, BE2, BE3, ISA1, ISA2, among others (see Supplementary File 1, sheet 3) when expressed in yeast. We selected multiple surrogate peptides for each protein and characterized the assays with respect to their dynamic range (Supplementary Files 1 and 2). Data calibration was supported using crude stable isotope labeled (SIL) peptides.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBarbara Pfister
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationListCarbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-21 11:58:08ID requested
12022-09-05 13:44:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: starch, heterologous expression, starch synthase, branching enzyme, debranching enzyme, isoamylase
Contact List
Samuel C. Zeeman
contact affiliationETH Zurich
contact emailszeeman@ethz.ch
lab head
Barbara Pfister
contact affiliationInstitute of Molecular Plant Biology, ETH Zurich
contact emailbpfister@ethz.ch
dataset submitter
Full Dataset Link List
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