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PXD033348 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA toolbox for systematic discovery of stable and transient protein interactors in baker’s yeast
DescriptionIdentification of both stable and transient interactions is essential for understanding protein function and regulation. While assessing stable interactions is more straightforward, capturing transient ones is challenging. In recent years, sophisticated tools have emerged to improve transient interactor discovery, with many harnessing the power of evolved biotin ligases for proximity labelling. However, biotinylation-based methods have lagged behind in the model eukaryote, Saccharomyces cerevisiae, possibly due to the presence of several abundant, endogenously biotinylated proteins. In this study, we optimised robust biotin-ligation methodologies in yeast and increased their sensitivity by creating a bespoke technique for downregulating endogenous biotinylation which we term ABOLISH (Auxin-induced BiOtin LIgase diminiSHing). We used the endoplasmic reticulum insertase complex (EMC) to demonstrate our approaches and uncover new substrates. To make these tools available for systematic probing of both stable and transient interactions, we generated five full-genome collections of strains in which every yeast protein is tagged with each of the tested biotinylation machineries; some on the background of the ABOLISH system. This comprehensive toolkit enables functional interactomics of the entire yeast proteome.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-21 06:49:32ID requested
12023-02-01 10:04:44announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: substrate discovery, TurboID, yeast libraries,interaction profiling
Contact List
contact affiliationDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel
contact emailmaya.schuldiner@weizmann.ac.il
lab head
contact affiliationWeizmann Institute of Science
contact emailmeital.kupervaser@weizmann.ac.il
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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