Update publication information. Protein phosphorylation is known to regulate a wide scenario of key cellular processes. However, phosphorylation mediated regulatory networks in honeybee embryogenesis are largely unknown. We identified 6342 phosphosites from 2438 phosphoproteins and predicted 168 kinases in the honeybee embryo. The worker and drone develop similar phosphoproteome architectures and central phosphorylation events during embryogenesis.