PXD033254

PXD033254 is an original dataset announced via ProteomeXchange.

Title	PhosphoProtein-Peptide Turnover Profiling, exploratory
Description	We combine pulsed stable isotope labeling of amino acids (pSILAC) with enrichment of phosphorylated peptides in a method we term PhosphoProtein-Peptide Turnover Profiling (PhosphoPPToP). We apply the method to HeLa cells to find peptides whose clearance rates differ from the protein median. Using theoretical modelling, we show that readouts from PPToP are primarily defined by the wiring of the PTM-modification network and not by effects of the PTM on a protein's proteolytic stability. Thus PPToP can be used to identify PTMs occurring, e.g., preferentially early or late in a protein's lifetime. This enables identification of, e.g., protein maturation or protein complex assembly intermediates.
HostingRepository	PRIDE
AnnounceDate	2022-12-02
AnnouncementXML	Submission_2022-12-02_11:47:59.826.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HenrikHammarén
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; isotope labeled residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 06:20:16	ID requested
⏵ 1	2022-12-02 11:48:00	announced

submitter keyword: post-translational modification, protein maturation, protein turnover, phosphorylation,Mass spectrometry

MikhailSavitski
contact affiliation	Genome Biology Unit and Proteomics Core Facility, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
contact email	mikhail.savitski@embl.de
lab head
HenrikHammarén
contact affiliation	Genome Biology Unit and Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
contact email	henrik.hammaren@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD033254

PRIDE project URI

• PRIDE
  1. PXD033254
     1. Label: PRIDE project
     2. Name: PhosphoProtein-Peptide Turnover Profiling, exploratory