Non-structural protein 13 (NSP13), as the helicase of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been shown to contain NTPase activity, DNA/RNA helicase activity, RNA 5′ triphosphatase activity and innate immunity antagonistic function. To study the function of SARS-CoV-2 NSP13, We detect the NSP13 interaction map in HEK293T cells by IP-MS.