PXD033024

PXD033024 is an original dataset announced via ProteomeXchange.

Title	The mitochondrial Hsp70 controls the assembly of the F1FO-ATP synthase
Description	The mitochondrial F1FO-ATP synthase produces the bulk of cellular ATP. The soluble F1 domain contains the catalytic head that is linked via the central stalk and the peripheral stalk to the membrane embedded rotor of the Fo domain. The assembly of the F1 domain and its linkage to the peripheral stalk is poorly understood. We uncovered a dual function of the mitochondrial Hsp70 (mtHsp70) in the formation of the ATP synthase. First, it cooperates with the assembly factors Atp11 and Atp12 to form the F1 domain of the ATP synthase. Second, the chaperone transfers Atp5 into the assembly line to link the catalytic head with the peripheral stalk. Inactivation of mtHsp70 leads to integration of an assembly-defective Atp5 variant into the mature complex, reflecting a quality control function of the chaperone. Thus, mtHsp70 acts as an assembly and quality control factor in the biogenesis of the F1FO-ATP synthase.
HostingRepository	PRIDE
AnnounceDate	2023-01-11
AnnouncementXML	Submission_2023-01-11_03:33:27.925.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JasjotSingh
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-06 08:55:10	ID requested
⏵ 1	2023-01-11 03:33:28	announced

Song J, Steidle L, Steymans I, Singh J, Sanner A, B, ö, ttinger L, Winter D, Becker T, -ATP synthase. Nat Commun, 14(1):39(2023) [pubmed]

submitter keyword: Mitochondria, F1FO-ATP synthase, Pulldown, mtHsp70

DominicWinter
contact affiliation	Institute for Biochemistry and Molecular Biology, Faculty of Medicine, University of Bonn
contact email	dwin@uni-bonn.de
lab head
JasjotSingh
contact affiliation	University of Bonn
contact email	jasjotsingh@web.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033024
     1. Label: PRIDE project
     2. Name: The mitochondrial Hsp70 controls the assembly of the F1FO-ATP synthase