we examined the label-free proteomics in untreated LNCaP (NC), DTP-ENZ, R5-ENZ, R10-ENZ, R20-ENZ, DTP-EPI, R5-EPI, R10-EPI, and R20-EPI groups by nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS). The groups each with three biological replicates were used for proteomics analysis.