PXD032868

PXD032868 is an original dataset announced via ProteomeXchange.

Title	Mouse apolipoproteome study - hyperlipidemic mouse models
Description	Atherosclerotic cardiovascular disease is the leading cause of death worldwide. For decades, mouse modelling of atherosclerosis has been the mainstay for pre-clinical testing of genetic and pharmacological intervention. Mouse models of atherosclerosis depend on supraphysiological levels of circulating cholesterol carried in lipoprotein particles. Lipoprotein particles vary in atherogenicity and it is critical to monitor lipoprotein levels during pre-clinical interventions in mice. Unfortunately, the small plasma volumes typically harvested during pre-clinical experiments limit analyses to measuring total cholesterol and -triglyceride levels. Here we developed a high-throughput, low-cost targeted multiple-reaction-monitoring (MRM)-based mass spectrometry assay for simultaneous measurement of nine apolipoproteins using few microliters of mouse plasma. We applied the MRM assay to investigate the plasma apolipoproteome of two atherosclerosis models: The widely used ApoE knockout model and the emerging recombinant adeno-associated virus-mediated hepatic Pcsk9 overexpression model. By applying the assay on size-exclusion chromatography-separated plasma pools we provide in-depth characterization of apolipoprotein distribution across lipoprotein species in these models, and finally, we use the assay to quantify apolipoprotein deposition in mouse atherosclerotic plaques. Taken together, we report development and application of an MRM assay with stable isotope dilution (SID) that can be adopted by fellow researchers to monitor the mouse plasma apolipoproteome during pre-clinical investigations.
HostingRepository	PanoramaPublic
AnnounceDate	2022-06-20
AnnouncementXML	Submission_2022-06-20_10:11:04.518.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Martin Overgaard
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-29 14:22:14	ID requested
⏵ 1	2022-06-20 10:11:05	announced

Steffensen LB, Larsen JH, Hansen DR, Tha TML, Larsen NS, Beck HC, Rasmussen LM, Overgaard M, Profiling the Plasma Apolipoproteome of Normo- and Hyperlipidemic Mice by Targeted Mass Spectrometry. J Proteome Res, 22(5):1385-1393(2023) [pubmed]

submitter keyword: MRM plasma mouse

Martin Overgaard
contact affiliation	Odense University Hospital, Dept. Clinical Biochemistry/SDU
contact email	martin.overgaard@rsyd.dk
lab head
Martin Overgaard
contact affiliation	Odense University Hospital, Dept. Clinical Biochemistry/SDU
contact email	martin.overgaard@rsyd.dk
dataset submitter

Panorama Public dataset URI