PXD032368

PXD032368 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	THRONCAT: Efficient metabolic labelling of newly synthesized proteins using a bioorthogonal threonine analogue
Description	Profiling the nascent cellular proteome and capturing early proteomic changes in response to external stimuli provides valuable insight into cellular physiology. Existing metabolic protein labelling approaches based on bioorthogonal methionine- or puromycin analogueues allow for the selective visualization and enrichment of the newly synthesized proteins, however, their applications are limited as they require methionine-free conditions or are toxic to cells. Here, we introduce a novel threonine-derived non-canonical amino acid tagging method, THRONCAT, based on bioorthogonal threonine analogueue β-ethynyl serine (βES) that enables efficient and non-toxic labelling of the nascent proteome in complete growth media within minutes. We used THRONCAT for the visualization and enrichment of nascent proteins in bacteria, mammalian cells, and drosophila melanogaster and rapidly profiled proteomic changes of Ramos B-cells in response to receptor activation in a time-stamp approach, demonstrating the potential and ease-of-use of the method.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:26.917.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jelmer Dijkstra
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-17 09:28:48	ID requested
1	2023-07-20 11:36:09	announced
⏵ 2	2023-11-14 09:03:30	announced	2023-11-14: Updated project metadata.

Publication List

Ignacio BJ, Dijkstra J, Mora N, Slot EFJ, van Weijsten MJ, Storkebaum E, Vermeulen M, Bonger KM, THRONCAT: metabolic labeling of newly synthesized proteins using a bioorthogonal threonine analog. Nat Commun, 14(1):3367(2023) [pubmed]

Ignacio BJ, Dijkstra J, Mora N, Slot EFJ, van Weijsten MJ, Storkebaum E, Vermeulen M, Bonger KM, THRONCAT: metabolic labeling of newly synthesized proteins using a bioorthogonal threonine analog. Nat Commun, 14(1):3367(2023) [pubmed]

Keyword List

submitter keyword: THRONCAT,BONCAT, LC-MS/MS

submitter keyword: THRONCAT,BONCAT, LC-MS/MS

Contact List

Michiel Vermeulen
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands
contact email	michiel.vermeulen@science.ru.nl
lab head
Jelmer Dijkstra
contact affiliation	RIMLS
contact email	jelmer.dijkstra@science.ru.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD032368
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD032368

PRIDE project URI

Repository Record List

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