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PXD032181 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePrediction and experimental validation of a new salinity-responsive cis-regulatory element (CRE) in tilapia
DescriptionQuantitative DIA proteomics in combination with the transcription inhibitor actinomycin D was performed on the tilapia OmB cell line to identify proteins that are upregulated by transcriptional regulation during hyperosmotic stress. This analysis revealed proteins that are transcriptionally up-regulated by hyperosmolality in these cells. The promoter regions of these proteins were compared and a novel hyperosmolality-induced cis-regulatory element (CRE) and corresponding transcription factor candidate were identified. The CRE was experimentlly validated by site-directed mutagenesis in combination with reporter assays.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDietmar Kültz
SpeciesList scientific name: Oreochromis mossambicus; NCBI TaxID: 8127;
ModificationListAcetyl; Carbamyl; Phospho; Dioxidation; Oxidation; Dehydrated; Methyl; Carbamidomethyl
Instrumentimpact II
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-09 21:19:07ID requested
12022-03-14 20:41:38announced
22022-09-25 22:12:29announced: Added PubMed Id
Publication List
Kim C, Wang X, K, ü, ltz D, Prediction and Experimental Validation of a New Salinity-Responsive Cis-Regulatory Element (CRE) in a Tilapia Cell Line. Life (Basel), 12(6):(2022) [pubmed]
Keyword List
submitter keyword: tilapia, OmB cell line, cellular osmoregulation, CRE, transcription factor, data-independent acquisition, quantitative proteomics
Contact List
Dietmar Kültz
contact affiliationUC Davis
contact emaildkueltz@ucdavis.edu
lab head
Dietmar Kültz
contact affiliationUC Davis
contact emaildkueltz@ucdavis.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI