PXD032181
PXD032181 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Prediction and experimental validation of a new salinity-responsive cis-regulatory element (CRE) in tilapia |
Description | Quantitative DIA proteomics in combination with the transcription inhibitor actinomycin D was performed on the tilapia OmB cell line to identify proteins that are upregulated by transcriptional regulation during hyperosmotic stress. This analysis revealed proteins that are transcriptionally up-regulated by hyperosmolality in these cells. The promoter regions of these proteins were compared and a novel hyperosmolality-induced cis-regulatory element (CRE) and corresponding transcription factor candidate were identified. The CRE was experimentlly validated by site-directed mutagenesis in combination with reporter assays. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2022-09-25 |
AnnouncementXML | Submission_2022-09-25_22:12:28.414.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Dietmar Kültz |
SpeciesList | scientific name: Oreochromis mossambicus; NCBI TaxID: 8127; |
ModificationList | Acetyl; Carbamyl; Phospho; Dioxidation; Oxidation; Dehydrated; Methyl; Carbamidomethyl |
Instrument | impact II |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-03-09 21:19:07 | ID requested | |
1 | 2022-03-14 20:41:38 | announced | |
⏵ 2 | 2022-09-25 22:12:29 | announced | : Added PubMed Id |
Publication List
Kim C, Wang X, K, ü, ltz D, Prediction and Experimental Validation of a New Salinity-Responsive Cis-Regulatory Element (CRE) in a Tilapia Cell Line. Life (Basel), 12(6):(2022) [pubmed] |
Keyword List
submitter keyword: tilapia, OmB cell line, cellular osmoregulation, CRE, transcription factor, data-independent acquisition, quantitative proteomics |
Contact List
Dietmar Kültz | |
---|---|
contact affiliation | UC Davis |
contact email | dkueltz@ucdavis.edu |
lab head | |
Dietmar Kültz | |
contact affiliation | UC Davis |
contact email | dkueltz@ucdavis.edu |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |