PXD032181

PXD032181 is an original dataset announced via ProteomeXchange.

Title	Prediction and experimental validation of a new salinity-responsive cis-regulatory element (CRE) in tilapia
Description	Quantitative DIA proteomics in combination with the transcription inhibitor actinomycin D was performed on the tilapia OmB cell line to identify proteins that are upregulated by transcriptional regulation during hyperosmotic stress. This analysis revealed proteins that are transcriptionally up-regulated by hyperosmolality in these cells. The promoter regions of these proteins were compared and a novel hyperosmolality-induced cis-regulatory element (CRE) and corresponding transcription factor candidate were identified. The CRE was experimentlly validated by site-directed mutagenesis in combination with reporter assays.
HostingRepository	PanoramaPublic
AnnounceDate	2022-09-25
AnnouncementXML	Submission_2022-09-25_22:12:28.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dietmar Kültz
SpeciesList	scientific name: Oreochromis mossambicus; NCBI TaxID: 8127;
ModificationList	Acetyl; Carbamyl; Phospho; Dioxidation; Oxidation; Dehydrated; Methyl; Carbamidomethyl
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 21:19:07	ID requested
1	2022-03-14 20:41:38	announced
⏵ 2	2022-09-25 22:12:29	announced	: Added PubMed Id

Kim C, Wang X, K, ü, ltz D, Prediction and Experimental Validation of a New Salinity-Responsive Cis-Regulatory Element (CRE) in a Tilapia Cell Line. Life (Basel), 12(6):(2022) [pubmed]

submitter keyword: tilapia, OmB cell line, cellular osmoregulation, CRE, transcription factor, data-independent acquisition, quantitative proteomics

Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
lab head
Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
dataset submitter

Panorama Public dataset URI