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PXD031945 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInfluence of immunoglobulin genotypes on the elicitation of SARS CoV-2 neutralizing antibodies
DescriptionThe human immunoglobulin heavy chain (IGH) locus is exceptionally polymorphic with high allelic diversity of variable (V) genes and structural variation affecting large regions of the locus. Thus, our germline IGHV gene and allele content is highly personal, which may influence how we respond to infections and vaccinations. Here, we coupled individualized IGHV genotyping with the isolation of monoclonal antibodies against the SARS-CoV-2 spike, focusing on the IGHV1-69 and IGHV3-30 group of genes, which were over-represented in spike-specific B cells. These genes are characterized by both allelic and copy number variations, making them ideal for expanding our understanding of inter-individual differences in antigen-specific antibody responses. We found that for the IGHV1-69*20-using CAB-I47 antibody, the allele usage was critical as germline reversion to other, highly similar, IGHV1-69 alleles abolished the neutralizing activity. Our results demonstrate that as little as single nucleotide differences between different alleles can greatly influence the biological response.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSimon Ekström
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-28 10:56:28ID requested
12023-03-11 02:45:36announced
22023-11-14 08:41:01announced2023-11-14: Updated project metadata.
Publication List
Pushparaj P, Nicoletto A, Sheward DJ, Das H, Castro Dopico X, Perez Vidakovics L, Hanke L, Chernyshev M, Narang S, Kim S, Fischbach J, Ekstr, ö, m S, McInerney G, H, ä, llberg BM, Murrell B, Corcoran M, Karlsson Hedestam GB, Immunoglobulin germline gene polymorphisms influence the function of SARS-CoV-2 neutralizing antibodies. Immunity, 56(1):193-206.e7(2023) [pubmed]
Keyword List
submitter keyword: Neutralizing antibody, SARS-CoV-2, Immunoglobulin germline allele, single nucleotide polymorphism, copy number variation,HDX-MS
Contact List
Gunilla B. Karlsson Hedestam
contact affiliationDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Box 280, S-171 77 Stockholm, Sweden.
contact emailGunilla.Karlsson.Hedestam@ki.se
lab head
Simon Ekström
contact affiliationBioMS - Swedish National Infrastructure for Biological Mass Spectrometry, Lund University, BMC D1330, 221 84 Lund, Sweden
contact emailsimon.ekstrom@med.lu.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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