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PXD031911 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpatial, Quantitative and Functional Deconstruction of Virus and Host Protein Interactions Inside Intact Cytomegalovirus Particles
DescriptionViral infections involve packaging of the viral genome and proteins into virions. Knowing virion composition and structure is critical to understand viral pathogenesis. However, an integrated picture of virion proteome organization of large viruses, such as Herpesviruses, is lacking. Here we use cross-linking mass spectrometry to derive a spatially resolved structural interactome of intact human cytomegalovirus virions. We capture interactions of 82 host and 33 viral proteins, allowing us to de novo allocate them into distinct virion-layers and identify several host proteins as constitutive virion components recruited via specific protein interactions. The abundant viral protein pp150 forms domain-specific interactions with all virion layers and scaffolds numerous viral and host proteins such as PP1 phosphatase and 14-3-3 proteins, which are incorporated via nearby short linear motifs in pp150’s C-terminus. PP1 recruitment antagonizes 14-3-3 proteins and is pivotal during early and late viral replication steps. Collectively, this study gives a spatial and quantitative inventory of the system-wide organization and functional relevance of protein interactions inside native herpesvirus particles.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Cytomegalovirus; NCBI TaxID: 10358; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human betaherpesvirus 5; NCBI TaxID: NCBITaxon:10359;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-28 01:10:04ID requested
12023-06-14 02:50:36announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cross-Linking, Virion,Herpesvirus
Contact List
contact affiliationLeibniz Forschungsinstitut für Molekulare Pharmakologie (FMP), Department of Structural Biology, Structural interactomics lab
contact emailfliu@fmp-berlin.de
lab head
contact affiliationLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
contact emailbogdanow@fmp-berlin.de
dataset submitter
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Dataset FTP location
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