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PXD031664 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLncRNA RUS shapes the gene expression program towards neurogenesis
DescriptionThe evolution of brain complexity correlates with an increased expression of long, non-coding (lnc) RNAs in neuronal tissues. Although prominent examples illustrate the potential of lncRNAs to scaffold and target epigenetic regulators to chromatin loci, only few cases have been described to function during neurogenesis. We present a first functional characterization of the lncRNA LINC01322, which we term RUS for ‘RNA upstream of Slitrk3’. The RUS gene is well conserved in mammals by sequence and synteny next to the neurodevelopmental gene Slitrk3. RUS is exclusively expressed in neural cells and its expression increases along with neuronal markers during neuronal differentiation of mouse embryonic cortical neural stem cells. Depletion of RUS locks neuronal precursors in an intermediate state towards neuronal differentiation, with arrested cell cycle and increased apoptosis. RUS associates with chromatin in the vicinity of genes involved in neurogenesis, most of which change their expression upon RUS depletion. The identification of a range of epigenetic regulators as specific RUS interactors suggests that the lncRNA may mediate gene activation and repression in a highly context-dependent manner.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephan Mueller
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-14 01:17:01ID requested
12022-06-21 14:35:12announced
Publication List
Schneider MF, M, ü, ller V, M, ü, ller SA, Lichtenthaler SF, Becker PB, Scheuermann JC, shapes the gene expression program towards neurogenesis. Life Sci Alliance, 5(10):(2022) [pubmed]
Keyword List
submitter keyword: RNA protein interaction
Contact List
Stefan F. Lichtenthaler
contact affiliationDZNE Munich Neuroproteomics Feodor-Lynen-Str. 17 81377 München Germany
contact emailstefna.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliationDZNE Munich Neuroproteomics
contact emailstephan.mueller@dzne.de
dataset submitter
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Dataset FTP location
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