PXD031189

PXD031189 is an original dataset announced via ProteomeXchange.

Title	The master energy homeostasis regulator PGC-1α couples transcriptional co-activation and mRNA nuclear export
Description	PGC-1α plays a central role in maintaining the mitochondrial and energy metabolism homeostasis, linking external stimuli to the transcriptional co-activation of genes involved in adaptive and age-related pathways. The carboxyl-terminus encodes a serine/arginine-rich (RS) region and a putative RNA recognition motif, however potential RNA-processing role(s) have remained elusive for the past 20 years. Here, we show that the RS domain of human PGC-1α directly interacts with RNA and the nuclear RNA export factor NXF1. Inducible depletion of endogenous PGC-1α and expression of RNAi-resistant RS-deleted PGC-1α further demonstrate that the RNA-binding activity is required for nuclear export of co-activated transcripts and mitochondrial homeostasis. Moreover, a quantitative proteomics approach confirmed PGC-1α-dependent RNA transport and mitochondrial-related functions, identifying also novel mRNA nuclear export targets in age-related telomere maintenance. Discovering a novel function for a major cellular homeostasis regulator provides new directions to further elucidate the roles of PGC-1α in gene expression, metabolic disorders, ageing and neurodegenerative diseases.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:08:33.218.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simeon Mihaylov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-24 02:43:58	ID requested
1	2023-09-06 15:49:48	announced
⏵ 2	2023-11-14 08:08:33	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: TMT LC-MSMS,Energy metabolism, Mitochondria, Gene expression, Peroxisome proliferator-activated receptor gamma coactivator 1 alpha, NXF1-dependent mRNA nuclear export

Guillaume M Hautbergue
contact affiliation	Sheffield Institute for Translational Neuroscience (SITraN), Department of Neuroscience, University of Sheffield, 385 Glossop Road, Sheffield S10 2HQ, United Kingdom
contact email	g.hautbergue@sheffield.ac.uk
lab head
Simeon Mihaylov
contact affiliation	The Francis Crick Institute
contact email	simeon.mihaylov@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD031189

PRIDE project URI

• PRIDE
  1. PXD031189
     1. Label: PRIDE project
     2. Name: The master energy homeostasis regulator PGC-1α couples transcriptional co-activation and mRNA nuclear export