Updated project metadata. The proteomes of three amphipod species, a Holarctic species Gammarus lacustris and two Biakal species Eulimnogammarus verrucosus and Eulimnogammarus cyaneus, were received by LC-MS/MS with TMT-labeling. All animals were collected in August 2018. Before the exposition, animals were acclimated in 6C for 25 days. Three species of amphipods were exposed to heat stress (24.6 C for 24 h) and control temperature (6C for 24 h).