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PXD030925 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRapidly evolving genes underlie Aedes aegypti mosquito reproductive resilience during drought
DescriptionFemale Aedes aegypti mosquitoes impose a severe global public health burden as primary vectors of multiple viral and parasitic pathogens. Under optimal environmental conditions, Aedes aegypti females have access to human hosts that provide blood proteins for egg development, conspecific males that provide sperm for fertilization, and freshwater that serves as an egg-laying substrate suitable for offspring survival. As global temperatures rise, Aedes aegypti females are faced with climate challenges, like intense droughts and intermittent precipitation, which create unpredictable and suboptimal conditions for the egg-laying step of their reproductive cycle. Aedes aegypti mosquitoes nonetheless show remarkable reproductive resilience, but how they achieve this is unknown. Here we show that under drought-like conditions simulated in the laboratory, mated, blood-fed Aedes aegypti females carrying mature eggs retain them in their ovaries for extended periods, while maintaining the viability of these eggs until they can be deposited in freshwater. Using transcriptomic and proteomic profiling of Aedes aegypti ovaries, we identify two previously uncharacterized genes – here named tweedledee and tweedledum – that show ovary-enriched, temporally-restricted expression during egg retention. These genes are mosquito-specific, linked within a syntenic locus, and rapidly evolving under positive selection, raising the possibility that they serve an adaptive function. Using loss-of-function mutagenesis to disrupt both genes, we show that, tweedledee and tweedledum, which encode secreted proteins, are specifically required for extended retention of viable eggs, such as during intermittent precipitation or drought. These results highlight an elegant example of taxon-restricted genes at the heart of an important adaptation that equips Aedes aegypti females with “insurance” to, when contextually appropriate, flexibly extend their reproductive sequence without losing reproductive capacity, thus allowing this species to exploit diverse and unpredictable/chaotic/changing habitats.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterhenrik molina
SpeciesList scientific name: Aedes aegypti; NCBI TaxID: 7159;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-13 01:35:33ID requested
12022-03-02 02:56:00announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Aedes aegypti
taxon-restricted genes
climate change
rapid evolution
Contact List
Leslie B. Vosshall
contact affiliationLaboratory of Neurogenetics and Behavior The Rockefeller University 1230 York Avenue Box 63 New York, NY 10065
contact emailleslie@rockefeller.edu
lab head
henrik molina
contact emailhenrik.molina@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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