PXD030824 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phospho- and total- proteomic analysis of FAK knockout in primary mouse lung endothelial cells |
Description | We assessed the impact of Endothelial Focal Adhesion kinase (ECFAK) knockout, using primary lung endothelial cells isolated (at P10-P14) from a FAK flox/flox mice crossed with mice expressing a tamoxifen-inducible Cre(iCreERT2) that is driven by endothelial cell (EC)-selective platelet-derived growth factor subunit B (Pdgfb) promoter (Pdgfb-iCreERT2) (Tavora et al., 2010). To induce EC restricted FAK deletion, we injected 4-hydroxytamoxifen (4-OHT) from postnatal day 1 (P1) to P2. Molecular signaling events modulated by ECFAK loss, with or without ex vivo VEGF stimulation, were then examined by phospho and total quantitative proteomics analysis using 6plex isobaric tandem mass tagging multiplexing (TMT). In total, three experiments were performed (a an initial pilot experiment not included in this study, followed by two main biological replicates included - i.e. experiments 2, and 3). The following 4 TMT channels were utilised in each experiment: TMT-126: WT-mock(PBS) stimulated TMT-127: WT-Vegf stimulated TMT128: FAK KO-mock (PBS)stimulated TMT 129:FAK KO-Vegf stimulated |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-13 |
AnnouncementXML | Submission_2022-04-13_03:42:50.803.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Faraz Mardakheh |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-07 13:37:40 | ID requested | |
⏵ 1 | 2022-04-13 03:42:51 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: FAK |
Endothelial Cells |
Quantitative proteomics |
VEGF |
TMT |
Contact List
Faraz Mardakheh |
contact affiliation | Centre for Cancer Cell & Molecular Biology Barts Cancer Institute, Queen Mary University of London Charterhouse Square London EC1M 6BQ U.K. |
contact email | f.mardakheh@qmul.ac.uk |
lab head | |
Faraz Mardakheh |
contact affiliation | Barts Cancer Institute |
contact email | f.mardakheh@qmul.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030824
- Label: PRIDE project
- Name: Phospho- and total- proteomic analysis of FAK knockout in primary mouse lung endothelial cells