<<< Full experiment listing


PXD030824 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhospho- and total- proteomic analysis of FAK knockout in primary mouse lung endothelial cells
DescriptionWe assessed the impact of Endothelial Focal Adhesion kinase (ECFAK) knockout, using primary lung endothelial cells isolated (at P10-P14) from a FAK flox/flox mice crossed with mice expressing a tamoxifen-inducible Cre(iCreERT2) that is driven by endothelial cell (EC)-selective platelet-derived growth factor subunit B (Pdgfb) promoter (Pdgfb-iCreERT2) (Tavora et al., 2010). To induce EC restricted FAK deletion, we injected 4-hydroxytamoxifen (4-OHT) from postnatal day 1 (P1) to P2. Molecular signaling events modulated by ECFAK loss, with or without ex vivo VEGF stimulation, were then examined by phospho and total quantitative proteomics analysis using 6plex isobaric tandem mass tagging multiplexing (TMT). In total, three experiments were performed (a an initial pilot experiment not included in this study, followed by two main biological replicates included - i.e. experiments 2, and 3). The following 4 TMT channels were utilised in each experiment: TMT-126: WT-mock(PBS) stimulated TMT-127: WT-Vegf stimulated TMT128: FAK KO-mock (PBS)stimulated TMT 129:FAK KO-Vegf stimulated
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFaraz Mardakheh
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-07 13:37:40ID requested
12022-04-13 03:42:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: FAK
Endothelial Cells
Quantitative proteomics
Contact List
Faraz Mardakheh
contact affiliationCentre for Cancer Cell & Molecular Biology Barts Cancer Institute, Queen Mary University of London Charterhouse Square London EC1M 6BQ U.K.
contact emailf.mardakheh@qmul.ac.uk
lab head
Faraz Mardakheh
contact affiliationBarts Cancer Institute
contact emailf.mardakheh@qmul.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD030824
PRIDE project URI
Repository Record List
[ + ]