Updated project metadata. This study is aimed to comprehensively and globally characterize seminal plasma glycoproteins in a site-specific manner with information of N-glycosites and corresponding glycan structures, as well as glycan isomers using mass spectrometry. Furthermore, the function of specific glycan structures is introduced during human reproduction. In addition, site-specific glycoproteins are compared precisely to explored the sub-glycan structure regulation of glycoprotein in human seminal plasma and spermatozoa.