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PXD030375 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic Analysis of Oil-Roasted Cashew Using a Customized Allergen-Focused Protein Database
DescriptionCashew is one of the most prevalent causes of tree nut allergies. However, the cashew proteome is far from complete, which limits the quality of peptide identification in mass spectrometric analyses. In this study, bioinformatics tools were utilized to construct a customized cashew protein database and improve sequence quality for proteins of interest, based on a publicly available cashew genome database. As a result, two additional isoforms for cashew 2S albumins and five other isoforms for cashew 11S proteins were identified, along with several other potential allergens. Using the optimized protein database, the protein profiles of cashew nuts subjected to different oil-roasting conditions (138 °C and 166 °C for 2-10 minutes) were analyzed using discovery LC-MS/MS analysis. The results showed that cashew 2S protein is most heat-stable, followed by 11S and 7S proteins, though protein isoforms might be affected differently. Preliminary target peptide selection indicated that out of the 29 potential targets, 18 peptides were derived from the newly developed database. In the evaluation of thermal processing effects on cashew proteins, several Maillard reaction adducts were also identified. The cashew protein database developed in this study allows for comprehensive analyses of cashew proteome and development of high-quality allergen detection method.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterShimin Chen
SpeciesList scientific name: Anacardium occidentale; NCBI TaxID: 171929;
ModificationListCarbamyl; Carbamidomethyl
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-13 16:20:22ID requested
12022-06-03 14:28:39announced
22022-09-25 22:20:35announced: Added PubMed Id
Publication List
Chen S, Downs ML, Proteomic Analysis of Oil-Roasted Cashews Using a Customized Allergen-Focused Protein Database. J Proteome Res, 21(7):1694-1706(2022) [pubmed]
Keyword List
submitter keyword: Cashew, Proteomics, Proteogenomics, Oil roasting, Allergen
Contact List
Melanie Downs
contact affiliationUniversity of Nebraska-Lincoln
contact emailmdowns2@unl.edu
lab head
Shimin Chen
contact affiliationUniversity of Nebraska-Lincoln
contact emailshimin@huskers.unl.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI