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PXD030348 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA proteomics analysis of 5xFAD mouse brain regions reveals the lysosome-associated protein Arl8b as a candidate biomarker for Alzheimers disease
DescriptionState-of-the-art mass spectrometry (MS) methods can comprehensively detect proteomic alterations in neurodegenerative disorders, providing relevant insights unobtainable with transcriptomics investigations. Analyses of the relationship between progressive aggregation and protein abundance changes in brains of 5xFAD transgenic mice have not been reported previously. We quantified progressive A? aggregation in hippocampus and cortex of 5xFAD mice and controls with immunohistochemistry and biochemical membrane filter assays. Protein changes in different mouse tissues were analysed by MS-based proteomics using label-free quantification (LFQ); resulting MS data were processed using an established pipeline. Results were contrasted with existing proteomic data sets from postmortem AD patient brains. Finally, abundance changes in the candidate marker Arl8b were validated in CSF from AD patients and controls using ELISAs. We report a comprehensive biochemical and proteomic investigation of hippocampal and cortical brain tissue derived from 5xFAD transgenic mice, providing a valuable resource to the neuroscientific community. We identified Arl8b, with significant abundance changes in 5xFAD and AD patient brains. Arl8b might enable the measurement of progressive lysosome accumulation in AD patients and have clinical utility as a candidate biomarker.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-13 02:55:41ID requested
12023-07-21 14:26:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mouse model, Amyloidogenesis,Proteomics, Alzheimers disease, 5xFAD
Contact List
contact affiliationNeuroproteomics, Max Delbrueck Center for Molecular Medicine in the Helmholtz Association, Robert-Roessle-Strasse 10, 13125 Berlin, Germany
contact emailerich.w@mdc-berlin.de
lab head
contact affiliationProteomics Platform, BIH@Charite
contact emailmarieluise.kirchner@mdc-berlin.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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